Spinach Chloroplastic Carbonic Anhydrase
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence of a cDNA encoding rice chloroplastic carbonic anhydrase.
CA (EC 4.2.1.1) catalyzes the reversible hydration of CO, according to the reaction CO, + H,O HCO, + H+ and represents 1 to 2% of total leaf soluble protein (Okabe et al., 1984). Considerable differences have been reported to exist between the molecular mass of the holoenzyme in monocotyledonous and dicotyledonous species (4245 kD in monocotyledonous plants and between 140 and 250 kD in dicotyl...
متن کاملRegulation of chloroplastic carbonic anhydrase : effect of magnesium.
It was previously reported that magnesium ion inhibited carbonic anhydrase (Bamberger and Avron 1975 Plant Physiol 56: 481-485). Studies with partially purified carbonic anhydrase from spinach (Spinacia oleracea L.) chloroplasts show that the effect was the result of the chloride counterion and not the magnesium ion. Enzyme activity was reduced 50% upon addition of 3 to 10 millimolar MgCl(2) or...
متن کاملZinc deficiency, carbonic anhydrase, and photosynthesis in leaves of spinach.
A shortage in the zinc supply to spinach (Spinacia oleracea L.) drastically reduced carbonic anhydrase levels with little effect on net CO(2) uptake per unit leaf area, except with the most severe zinc stresses. Under these conditions, carbonic anhydrase was below 10% and photosynthesis 60 to 70% of the control levels. When photosynthesis was measured at a range of CO(2) supply levels, zinc-def...
متن کاملIsolation and characterization of a cDNA coding for pea chloroplastic carbonic anhydrase.
Using a polyclonal antibody generated against the purified pea (Pisum sativum) carbonic anhydrase (CA) monomeric species, we have isolated and characterized a cDNA coding for this enzyme. Protein sequence analysis was used to confirm the identity of the clone. The presence of a large transit peptide suggests that CA is transported into the chloroplast and then processed to the mature size of ap...
متن کاملCarbonic anhydrase under pressure
Investigations of the rapid enzyme carbonic anhydrase have now been extended by crystallographic analysis at high CO2 pressures to examine the movements of water molecules in different steps of the catalysis. The rate of catalysis seems well explained by the assembled observations.
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1990
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.92.1.37